Role of mitochondrial YAP and TAZ in muscle growth, regeneration and mechanotransduction

Projet de stage : YAP and TAZ are transcriptional co-regulators sensing cell contractility and promoting tissue growth through the expression of nuclear target genes involved in cell cycle and survival. We have preliminary evidence that YAP and TAZ are also localized in the mitochondria. The student will use imaging, metabolomics and biochemical purification to analyze the role and regulation of mitochondrial YAP/TAZ. These studies may suggest a powerful mechanism in the cell to coordinate metabolic responses in nucleus and mitochondria. Mechanical cues could therefore be transduced by mitochondrial YAP/TAZ in order to stimulate ATP production for cell contractility. Techniques mises en œuvre par le stagiaire : confocal immunofluorescence, metabolomic analysis, purification of mitochondria, gene expression