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> Stages de M2 > Liste des stages proposés pour l’année 2011-2012 > Phosphorylation des ribosomes et synthèse proteique

Phosphorylation des ribosomes et synthèse proteique

proposé par Mario Pende, INSERM, CHU Necker, Paris

Projet de stage : The phosphorylation of ribosomal protein S6 (rpS6) has been described long time ago in response to growth factors and nutrient availability, though the functional relevance is yet unknown. The kinases responsible for this modification have been identified as the S6 kinases (S6K1 and S6K2). Since genetic inactivation of S6 kinases in flies and mice leads to growth defects, it is possible that the phosphorylation of ribosomes participates in organismal growth. To address this question, we will analyse mutant mice and cells carrying loss-of-function mutations in S6 kinases, as well as non-phosphorylatable mutants of rpS6. The students will perform the following experimental approaches : 1) polysome fractionation followed by QRTPCR or microarray analysis to identify the classes of mRNAs under the control of rpS6 phosphorylation ; 2) analysis of ribosome biogenesis ; 3) rescue experiments to re-establish the expression of downstream targets and demonstrate the functional relevance. These studies should have broad implication in signal transduction, translational control, cell growth and tumorigenesis

Techniques mises en œuvre par le stagiaire : polysome fractionation, QRTPCR, biochemistry, molecular biology

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